Welcome to `fba`’s documentation!¶

fba: a flexible and streamlined package for feature barcoding assays.

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Maintainability

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Quickstart¶

Installation¶

$ pip install fba

Usage¶

$ fba

usage: fba [-h]  ...

Tools for feature barcoding analysis

optional arguments:
-h, --help        show this help message and exit

functions:

   extract         extract cell and feature barcodes
   map             map enriched transcripts
   filter          filter extracted barcodes
   count           count feature barcodes per cell
   demultiplex     demultiplex cells based on feature abundance
   qc              quality control of feature barcoding assay
   kallisto_wrapper
                  deploy kallisto/bustools for feature barcoding
                  quantification

Citation¶

Jialei Duan, Gary Hon. FBA: feature barcoding analysis for single cell RNA-Seq. Bioinformatics. 2021 May 17:btab375. doi: 10.1093/bioinformatics/btab375. Epub ahead of print. PMID: 33999185.